A rapid method for embedding tissues for electron microscopy using 1,4-dioxane and Polybed 812.
نویسندگان
چکیده
منابع مشابه
An Embedding Technique for Electron Microscopy Using Epon 812
E p o x y resins h a v e been es tab l i shed as des i rab le e m b e d d i n g m e d i a for e lec t ron m i c r o s c o p y (1-5) . F inck (6) desc r ibed a n epoxy resin, E P O N 812 (Shell) t , w h i c h in a d d i t i o n to its o t he r des i rab le charac te r i s t ics , is also a p p r e c i a b l y misc ib le w i th wate r . H e no t ed t h a t p r o l o n g e d a lcohol d e h y d r a...
متن کاملA routine method for embedding animal tissues in Spurr resin for electron microscopy.
The epoxy resin epon 812 has been used in this department to embed a variety of normal and pathological tissues for a number of years. Unfortunately during the past 18 months sectioning qualities of polymerized epon blocks have become variable in an unpredictable manner. It is possible that the batch variation in the quality and composition of the dodecynl succinic anhydride (DDSA) could be res...
متن کاملpassivity in waiting for godot and endgame: a psychoanalytic reading
this study intends to investigate samuel beckett’s waiting for godot and endgame under the lacanian psychoanalysis. it begins by explaining the most important concepts of lacanian psychoanalysis. the beckettian characters are studied regarding their state of unconscious, and not the state of consciousness as is common in most beckett studies. according to lacan, language plays the sole role in ...
Problems in Methacrylate Embedding for Electron Microscopy
During an investigation of the effect of ischemia on striated muscle as seen with the electron microscope it became evident that all degrees of disorientation and destruction of tissues were taking place, and it seemed likely that much of the damage occurred during the embedding process. Such damage made it difficult to proceed with experiments in which comparison of normal tissue with any path...
متن کاملA REPORT OF RAPID AND EFFICIENT METHOD FOR MITOCHONDRIAL DNA
Based on the similarity of prokaryotic DNA to that of mitochondrial DNA, a rapid and efficient method for DNA purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by Marmur (1983) which resulted in a high degree of purity (A260/A280=2). In addition we have applied the same technique for the first time to purify...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1980
ISSN: 0022-1554,1551-5044
DOI: 10.1177/28.5.6892923